From: Subject: Non-Volatile Factor Determination Calculation Date: Thu, 15 May 2008 16:07:21 -0400 MIME-Version: 1.0 Content-Type: text/html; charset="iso-8859-1" Content-Transfer-Encoding: quoted-printable Content-Location: mhtml:file://C:\Documents and Settings\webtech\Local Settings\Temporary Internet Files\OLKF9\Technique - Non-Volatile Det'n EPA-1.mht X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.3198 Non-Volatile Factor = Determination Calculation

 

 

Non-Volatile=20 Factor Determination Calculation

for = Pressurized=20 Containers (Aerosol Cans)

 

 

  1. Remove all = loose items=20 (labels, caps, nozzles, etc.) and weigh entire can - Gross = Weight=20

 

  1. Freeze = (overnight),=20 place in metal beaker and puncture top of can with ice pick, 10 penny = nail, or=20 awl allowing can to Avent@=20 and all come to room temperature. Open or remove top with can opener=20 (mechanical or manual) being careful of sharp metal edges.

 

  1. Re-weigh can = containing=20 remaining liquid - Thaw Weight

 

  1. Transfer = liquid to=20 appropriate container and retain for later assay/analysis.

 

  1. Wash, clean, = dry, and=20 weigh empty can (woth any items from inside) - Empty Weight =

 

 

Calculate Non-Volative Factor as = follows:

 

         =20   (Thaw Net = Weight) / (Gross Net Weight) =3D NVF

 

 

Where: Gross Net Weight =3D Gross Wt. less Empty = Wt.

 

Thaw Net Wt. =3D Thaw Wt. Less Empty Wt.

 

NVF =3D Non-Volatile Factor 

 

 

Using NVF in Calculations for % Active Ingredients = :

 

         =20 % A I  =3D  (R/R=3D)=20 x (W=3D/W)=20 x P x NVF

 

Where: % A I =20 =3D  percent active = ingredient

R  =3D =20 peak height/area response of A I in sample

R=3D  =3D =20 peak height/area response of A I in standard

W=3D=20 =3D  concentration of A I = in standard=20 as mg/ml

W =3D  concentration of sample as = mg/ml

P  =3D =20 purity of standard

NVF  =3D =20 non-volatile factor   =20