ERP Adopts First Action Methods for Flavanols
An AOAC expert review panel (ERP), chaired by James Harnly of the U.S.
Department of Agriculture, granted First Action status to two methods for
flavanols in foods. Methods were evaluated against standard method performance
requirements (SMPRs) developed and approved through volunteer stakeholder
On March 14, 2013, during the AOAC Mid-Year Meeting, the ERP agreed that the
method, “Modification to OMA 2012.24 Analysis of Cocoa Flavanols and
Procyanidins (DP 1-10) in Cocoa-Containing Ingredients and Products by Rapid
Resolution Liquid Chromatography: A Single-Laboratory Validation,” submitted by
Mars, meets SMPRs as developed and approved by the Stakeholder Panel on
Strategic Foods Analytical Methods (SPSFAM). The method is codified as First
Action Official MethodSM 2013.03.
The results of the SLV were compared to the results previously obtained in
the collaborative study of 2012.24, and found to be consistent. The
translation to rapid resolution LC allowed for an 80% reduction in analysis time
and solvent usage for 2013.03, while retaining the accuracy and
reliability of the original method.
The ERP also granted First Action status to the method, “Determination of
Catechin and Epicatechin Enantiomers in Cocoa-Based Ingredients and Products by
High-Performance Liquid Chromatography: Single-Laboratory Validation,” also
submitted by Mars [J. AOAC Int. 95, 500(2012)]. Results of the method
show suitable performance for routine laboratory use in analysis of cocoa-based
products. In an SLV study, the method was able to identify and quantify the
flavanol enantiomers (+)- and (-)-epicatechin and (+)- and (-)-catechin in
cocoa-based ingredients and products. These compounds were eluted isocratically
with an ammonium acetate-methanol mobile phase applied to a modified
ß-cyclodextrin chiral stationary phase and detected using fluorescence. The
method was approved as First Action Official MethodSM
Full coverage appears in the March/April 2013 issue of Inside Laboratory
Management. The newly approved methods for flavanols, as well as the SMPR,
are available on the Official Methods of Analysis Online (www.eoma.aoac.org) and are
currently in process for publication in the Journal of AOAC
The next SPSFAM meeting will be held August 23, 2013, prior to the AOAC
Annual Meeting and Exposition in Chicago, Illinois, USA.
For more information on SPSFAM, of if you would like to participate, visit http://www.aoac.org/SPSFAM/spsfam.htm or contact Dawn Frazier,
executive for scientific business development, at firstname.lastname@example.org.