SCIENTIFIC SESSIONS
Sunday, September 26, 2010
4:00 pm - 5:30 pm
BP Oil Spill: Government Impact Assessment, Food Safety Issues, the Analytical Challenges and Method Development
Monday, September 27, 2010
1:30 pm - 3:00 pm
Wiley Award Symposium: Testing for Payment, Quality, and Adulteration: Is It All Hidden in the Mid-IR Spectra?
Best Practices for the Determination of Small Molecule Contaminants in Foods
Novel Molecular Methods for Detection and Identification of Pathogens
3:30 pm - 5:00 pm
MY WAY is Best! Microbiology Performance Criteria as Defined per Regulatory Agencies
TDLM Workshop: Traceability - Tested Once, Accepted Everywhere
New Trends in Food Residue Analysis with "New Tech" Mass Spectrometers (Targeted/Non-Targeted Analysis)
Tuesday, September 28, 2010
8:15 am - 9:45 am
Non-Targeted Analysis Strategies for Food Safety
A Promising LC/MS/MS Allergen Detection Alternative: Are We There Yet?
Marine Toxin Control: Modern Methods, Validations, Animal Use Reduction, and International
Standards
10:15 am - 11:45 am
Emerging Technologies for Non-Targeted Residue and Contaminant Analysis
Detection Issues and Criteria for Validation of Analytical Methods for Cyanotoxins Detection and Global Suitability
Roundtable on Gluten Detection: Achievements or Else?
11:45 am - 3:00pm
No Scientific Sessions. Please use this time to visit the Exhibit Hall.
3:00 pm - 4:30 pm
Roundtable on Pesticide Formulations: Analytical Challenges, Regulatory Issues, Plus Unique Analyses
TDRM Symposium: Use of Reference Materials and Resources
Natural Toxins: Impact and Methods Needs in the Developing World
Wednesday, September 29, 2010
8:15 am - 9:45 am
New Blood: Developing Methods for the Detection of Chemical Contaminants
Hot Topic Symposium: Food Fraud and Food Authenticity: How to Ensure to Get Your Money's Worth
8:15 am – 11:45am
Chemical Authentication of Botanicals Using Chromatographic and Spectral Fingerprinting Methods
10:15 am - 11:45 am
I Want to Use a New Microbiology Method: How Do I Validate Methods for Use in My Own Lab?
TDLM Symposium: Why Did That Happen? And How Do I Fix It?
Root Cause Analysis and CAPA as Told by Those Who Live It Every Day in the Lab
1:00 pm - 2:30 pm
New Validation Strategies for the 21st Century
Disinfectants: Exotica Redux
Harnessing Information: Digital and Analog Approaches to Pathogen Detection
3:00 pm - 4:30 pm
Analytical Challenges: Mycotoxins and Food Safety
Phytochemical Analysis of Herbal Raw Materials and Regulatory Challenges
XRF for Screening of Foods and Nutritional Supplements; Capabilities, Use and Limitations
Sunday, September 26, 2010
4:00 pm - 5:30 pm
BP Oil Spill: Government Impact Assessment, Food Safety Issues, the Analytical Challenges and Method Development
CO-CHAIR: Jo Marie Cook, Chief, Bureau of Chemical Residue Laboratories, Florida Department of Agriculture and Consumer Services
CO-CHAIR: John M. Reuther, Laboratory Director, Eurofins CAL
Jo Marie Cook, Florida
Department of Agriculture and Consumer Services
Analytical Methodologies Used in Seafood Contaminant Assessment
Edward B. Overton, Louisiana State University
Chemical Overview of the Deepwater Horizon Oil Spill
Michael A. McLaughlin, U.S. FDA - ORA
FDA’s Response to the Oil Spill: Assuring Food Safety
Bob Jones, Southeastern Fisheries Association
Oil Spill Impact on the Gulf Shore’s Seafood Industry
Monday, September 27, 2010
1:30 pm - 3:00 pm
Wiley Award Symposium: Testing for Payment, Quality, and Adulteration: Is It All Hidden in the Mid-IR Spectra?
The Wiley Award Symposium will provide an example of how method validation, proficiency testing, and preparation of reference materials has been utilized in the dairy industry over the past 25 years to improve the accuracy of milk payment testing, dairy cattle feeding management and genetic selection, and to control of dairy product composition. In addition, cutting edge applications of mid-infrared milk analysis to predict fatty acid composition of milk and to rapidly screen milk for adulterants using chemometric analysis of mid-infrared spectra will be addressed.
CHAIR: Cary Hunter, Market Administrator, USDA, AMS, Dairy Programs, Market Administrator's Office
David Mark Barbano, Cornell University, Department of Food Science
An Overview of the Development, Evolution, and Implementation of High Speed Mid-IR Milk Analysis
Evert A.M. de Jong, Delta Instruments B.V.
Profiling and the Monitoring of Fatty Acid Characteristics of Milk Fat by Routine Mid-IR Milk Analysis
Per Waaben Hansen, FOSS Analytical A/S
Screening for Adulteration of Milk Using Fourier Transform Infrared (FT-IR) Spectroscopy
Best Practices for the Determination of Small Molecule Contaminants in Foods
Chemical residue analysis in food extracts is a complex task requiring the analyst to search for dozens, or even hundreds of compounds in a wide variety of matrices. Pesticide residues, mycotoxins, bisphenol A, melamine, and acrylamide are just some of the contaminants targeted by modern food laboratories. Traditional methods have focused on a short list of target compounds, but many labs recognize the need to combine broad screening approaches with better techniques for target compounds. This session focuses on the best mass spectral tools and techniques for broad screening and targeted compound analysis. The emphasis is on techniques that use proven technology rather than more experimental methods that are difficult to implement in a real laboratory setting. The session will be of interest to those who want to modernize their chemical residue methods in order to achieve lower detection limits and screen for many more compounds.
CO-CHAIR: Philip L. Wylie, Senior Applications Chemist, Agilent Technologies, Inc.
CO-CHAIR: Peter J. W. Stone, Senior Applications Chemist, Agilent Technologies, Inc.
Gregory E. Mercer, US FDA - ORA
Targeted GC Pesticide Analysis at FDA: Transitioning to GC/MS/MS from GC/MS with Selected Ion Monitoring
Jack C.Cappozzo, National Center for Food Safety & Technology
Analysis of Mycotoxins and Pesticides by LC/QQQ
Katerina Mastovska, Covance Laboratories
Use of GC-MS/MS for Routine Pesticide Residue Screening and Quantitation
Immaculada Ferrer, University of Colorado
Large Screening and Analysis of Unknown Compounds in Foods by LC/TOF/MS
Novel Molecular Methods for Detection and Identification of Pathogens
For more than a hundred years, conventional microbiological methods employing culture media have been the mainstay of food analysts conducting biological tests for pathogens. Molecular biology methods, based on nucleic acid and antibody-based technologies represent a new generation of assays that provide significantly enhanced sensitivity and specificity over conventional microbiological methods. The food testing industry is now witnessing a very rapid conversion from microbiological to molecular-based testing technologies. Each of the talks in this session will describe new molecular-based testing technologies and will focus on the advancements in pathogen detection, identification and characterization afforded by these novel approaches.
CO-CHAIR: Mary T. McBride, Director, Government Relations, Agilent Technologies, Inc.
CO-CHAIR: Brooke Schwartz, Senior Director, Food and Environmental Testing, Applied Biosystems/Life Technologies
Robert Tebbs, Applied Biosystems/Life Technologies
Novel Molecular Methods for Pathogen Detection
Craig Parker, USDA - ARS
Molecular-Based Assays for the Detection and Differentiation of STECs
Eric W. Brown, U.S. FDA - CFSAN
Sequence-Based Subtyping and the Systematic Search for Solutions to Salmonella
Gregory Richmond, Agilent Technologies, Inc.
MassCode Liquid Array Enables Molecular Serotyping of Salmonella
Lisa Gorski, USDA - ARS
Determining the Optimal Microbiological Conditions for the Molecular Detection of Salmonella from
Contaminated Produce
3:30 pm - 5:00 pm
MY WAY is Best! Microbiology Performance Criteria as Defined per Regulatory Agencies
There has been much turmoil in the global food industry over the last few years: numerous national food-borne outbreaks, administration changes, food safety legislation, new and improved rapid methods, and efforts towards global methods validation and harmonization, etc. As a result of these constant changes, methods and the processes for method validation by regulatory agencies has also evolved.
Where are we at today? Has each agency defined microbiological performance criteria that can be followed? What is expected to happen, based on the current regulatory/legislative climate?
CO-CHAIR: DeAnn L. Benesh, Regulatory Affairs Manager, 3M
CO-CHAIR: Michelle Iannucci, Associate Principal Scientist, Kraft Foods, USA
Thomas S. Hammack, US FDA - CFSAN
Microbiological Methods Validation from FDA's Perspective
Peter Evans, USDA - FSIS
Microbiological Methods Validation from USDA's Perspective
Sasitorn Kanarat, Department of Livestock Development, Thailand
DLD's Perspective on Microbiological Performance Criteria
Paul H. In't Veld, Food and Consumer Product Safety Authority
Dutch Food Safety Authority: Method Validation, European Perspective
TDLM Workshop: Traceability - Tested Once, Accepted Everywhere
The use of a traceable reference material is not all there is to establishing traceability. One must adequately identify and control all influence quantities that significantly affect the result. Is your lab prepared to do this? Traceability concepts will be explained so they are understandable and palatable. The workshop participants will then work on real life examples with group discussions. Answers for the examples will be distributed. To achieve comparability of results over space and time, it is essential to link all the individual measurement results to some common, stable reference or measurement standard. Results can be compared through their relationship to that reference. This strategy of linking results to a reference is termed “traceability.” After the presentations, real life examples will be presented for you to work through, either with a facilitator or on your own. There will be limited seats at the tables where the facilitators will help people work through the real life examples, so arrive early to ensure you get a seat at a table with a facilitator.
CHAIR: M.L. Jane Weitzel, Director, Laboratory Services, Biovail Corporation
Franz Ulberth, European Commission – Institute for Research Materials and Measurements
Metrological Traceability of Analytical Results: Basic Concepts and Principles
Robert Watters, NIST
Traceability for Chemical Measurements: The Meaning of Calibration
New Trends in Food Residue Analysis with "New Tech" Mass Spectrometers (Targeted/Non-Targeted Analysis)
Over the past 10 years, an unprecedented evolution has occurred in Food Analytical Laboratories. Cumbersome and time consuming techniques are being replaced by powerful tools enhancing accuracy, selectivity and productivity of tests. Inside the Research Laboratories of Chromatography Companies, Contract and State Laboratories, new stationary phases, new equipments and new methods have evolved like Fast Resolved LC
(UPLC®), Triple Quad MS’s, Time of Flight MS’s, Linear/Orbital Traps MS’s, and Hybrid MS Systems such as Q-TOF and LTQ-Orbitrap instruments. The symposium dedicated to “New trends in Food Residue Analysis” will present some of the most advanced findings in Food Residue Research, proposed by top speakers in the field. For demonstration, amongst the areas covered by chemical contaminants in food, two key topics have been selected: Drug Residues and Pesticide Contaminants. The symposium session is aimed at laboratory managers as well as researchers using LC techniques to provide an update on recent developments as well as forming a discussion platform between scientists involved in contract, academic and public laboratories.
CO-CHAIR: Eric Verdon, Head of Veterinary Drug Residue Department, Laboratory for Study and Research on Veterinary Drugs and Disinfectants, ANSES, the French Agency for Food, Environmental and Occupational Health Safety, Laboratory of Fougeres
CO-CHAIR: Jo Marie Cook, Chief, Bureau of Chemical Residue Laboratories, Florida Department of Agriculture and Consumer Services Division of Food Safety
Walter T. Hammack, Florida Department of Agriculture and Consumer Services
The Application of High Resolution and Accurate Mass for the Determination of Target and Non-Target
Compounds in Food
Richard Fussell, Food and Environment Research Agency (Fera)
Ambient Ionization Coupled to Hybrid Mass Spectrometers: A New Technique for Food Quality/Safety Control
Sherri B. Turnipseed, U.S. FDA - ADRC
Quadrupole Time-Of-Flight LC-MS Analysis of Veterinary Drug Residues in Milk Samples
Jian Wang, Canadian Food Inspection Agency
Applications of LC-esi-MS/MS, UHPLC QqTOF MS and UHPLC Orbitrap for the Determination of 150 Pesticides in Food
Eric Verdon, ANSES of Fougeres
The "LTQ-ORBITRAP": An Instrument Dedicated to Multi-Functional Search in Molecular Chemistry
Tuesday, September 28, 2010
8:15 am - 9:45 am
Non-Targeted Analysis Strategies for Food Safety
Expecting the unexpected appears to be the only strategy that can ensure food safety. Just as with quality in manufacturing, testing alone cannot guarantee a safe and high quality food product. However, in conjunction with a sensible decision-making scheme, non-targeted analysis can provide valuable information to inform critical decisions about the food that reaches our plates. Whether it is a general unknown screen, food profiling, identification of new toxins, or a comprehensive residue analysis, non-targeted analysis is a powerful tool that can aid agencies and food producers alike. This session will discuss the appropriate application of all these strategies by experts in the field.
CO-CHAIR: Alexander J. Krynitsky, Chief, Bioanalytical Methods Branch, U.S. FDA - CFSAN
CO-CHAIR: James Chang, Thermo Scientific
Adrian J. Charlton, The Food and Environment Research Agency
Non-Targeted Detection of Chemical Contaminants in Food and Beverages by Nuclear Magnetic Resonance Spectroscopy and High-Resolution Mass Spectrometry
Jana Hajslova, Institute of Chemical Technology, Prague
Ambient Mass Spectrometry with Ultra High Resolution Mass Spectrometry as a Powerful Tool for Rapid
Profiling of Food
Sneh D. Bhandari, Silliker Laboratory
Strategies in Screening of Targeted and Non-Targeted Contaminants in Food
Jon W. Wong, U.S. FDA - CFSAN
Pushing the Limits of Pesticide Analysis
Oral Poster Presentation:
Michal Godula, Thermo Fisher Scientific
A Novel High-Resolution Mass Spectrometry Strategy for the Non-Targeted Detection of Chemicals in Food
A Promising LC/MS/MS Allergen Detection Alternative: Are We There Yet?
Quantitative detection of food allergens in the food chain is a strategic food safety objective to ensure safe food for all consumers. While ELISA development has matured, alternative methods are lagging behind. Progress in mass spectrometry has greatly opened up the field of proteomics. These advances are now available for the detection and the quantification of traces of allergenic proteins in complex mixtures using either a bottom-up or top-down approach. This session will explore the recent advances in quantitative LC/MS/MS for food allergens. So, are we there yet? Stay tuned.
CO-CHAIR: Jupiter M. Yeung, Principal Scientist, Nestlé Gerber
CO-CHAIR: Samuel Benrejeb Godefroy, Director General, Food Directorate, Health Canada
Petra Lutter, Nestlé Research Center
Quantification of Milk Traces in Food Matrices Using Isotope Dilution Liquid Chromatography-Mass
Spectrometry
Julia Heick, Eurofins Analytik GmbH
Progress in Mass Spectrometric Analysis of Food Allergens
Antonietta Gledhill, Waters Corporation
The Analysis of Allergens in Raw and Roasted Peanuts Using UPLC-QT of MS
Terry B. Koerner, Health Canada
The Tale of Two Beers: Mass Spectrometry and Gluten Sensitivities
Oral Poster Presentation:
Sigrid Haas-Lauterbach, R-Biopharm AG
RIDASCREEN® Gliadin Competitive - Second Generation (Testing for Gliadin in Compliance with Codex
Alimentarius Level)
Marine Toxin Control: Modern Methods, Validations, Animal Use Reduction, and International
Standards
This session will address major developments now in play for the monitoring of shellfish for marine biotoxins: Initiatives to move away from animal-based monitoring, continuing advances in mass-based detection techniques, and the completion of multiple interlaboratory validation studies. Due to both performance and ethical issues, Europe has committed to trying to replace the current mouse bioassays for lipophilic shellfish toxins with alternative chemical methods within two years, if validation efforts succeed. LC-MS methodology continues to achieve higher performance levels and yet the requirement for rugged methods, particularly for the lipophilic toxins, must also be met to support their use in monitoring. Efforts addressing other shellfish toxins, particularly the paralytic shellfish toxins (saxitoxins) continue to produce animal use reductions, more training, and new validation studies. All of these efforts, which are expected to improve food safety while supporting international trade, also depend on the availability of reference materials and harmonized method validation requirements.
CO-CHAIR: Ana Gago-Martinez, Professor, Universidad de Vigo
CO-CHAIR: Lei Bao, Director, Shan Dong Import-Export Inspection and Quarantine Bureau of China
Takeshi Yasumoto, Okinawa Science and Technology Promotion Center
Monitoring Methods and Analytical Tools for Lipophilic Marine Toxins: From Bioassays to LC-MS/TOF
Paolo Caricato, European Commission - EU Commission Official
Moving from Mouse Bioassays to Alternative Methods for Marine Toxins in Shellfish: European Perspective
Frances M. van Dolah, NOAA - National Ocean Service
Determination of Paralytic Shellfish Poisoning Toxins in Shellfish by Receptor Binding Assay: A
Collaborative Study
Betsy Jean Yakes, U.S. FDA - CFSAN
Surface Plasmon Resonance Biosensors for Detection of Tetrodotoxin in Food Matrices and Comparison to Analytical Methods
10:15 am - 11:45 am
Emerging Technologies for Non-Targeted Residue and Contaminant Analysis
The recent food safety scares that have hit the Global Food community have highlighted that there is a need for change within the current laboratory protocols that are used as these reactive approaches focus on the development of new methods to target new contaminants after the event. The industry trend is to establish proactive initiatives/analytics that allow scientists to not only perform targeted analysis of known residues and chemical contaminants (such as pesticides, mycotoxins, packaging migration products, allergens) but also allow untargeted compounds (emerging chemical contaminants / residues) to be analyzed and identified. In this session our speakers will discuss their strategies to address screening of targeted and untargeted food contaminants using new technologies and workflow management tools for the laboratory.
CO-CHAIR: Joe Romano, Senior Manager, Chemical Analysis, Waters Corporation
CO-CHAIR: Jonathan W. DeVries, Sr. , Senior Technical Manager, Medallion Laboratories/General Mills
Walter T. Hammack, Florida Department of Agriculture and Consumer Services
Strategies for the Detection and Identification of Unknown Contaminants in Food Products
John Szpylka, Medallion Laboratories/General Mills
Approaches of Monitoring to Assure Ingredient Authenticity
Antonietta Gledhill, Waters Corporation
Targeted and Non-Targeted Analysis of Chemical Contaminants in Food, Environmental and Wildlife Matrices
Detection Issues and Criteria for Validation of Analytical Methods for Cyanotoxins Detection and Global Suitability
Cyanotoxins have emerged as an issue of worldwide concern due to known acute and chronic toxicity affecting human and ecological health. Harmonization and validation of laboratory sample processing and analytical methodology for many classes of toxins such as anatoxins, cylindrospermopsins, microcystins, and saxitoxins are still needed to scientifically assess cyanotoxin occurrence, fate, transport, and effects which are essential aspects of risk assessment. A number of technologies have been developed for both laboratory and field quantitation of cyanotoxins that include a variety of chromatographic techniques as well as biological assays such as immunoassay-based platforms (e.g. tube, strip, and microplate formats). All of these methods are affected to varying degrees by the inadequacy of commercially available certified reference materials of known purity and stability and matrix effects, and many require sample pre-concentration to reach acceptable minimum reporting limits. It has become obvious that methods validation must include a variety of matrices. Analytical methods validation criteria should be flexible enough to encompass multiple environmental matrices and yet provide scientifically defensible data. This session will provide the perspective of developing nations on cyanotoxin occurrence and methods validation, and propose validation criteria for further discussion by the Marine and Freshwater Toxin Taskforce.
CO-CHAIR: Armah de La Cruz, Research Microbiologist, U.S. EPA
CO-CHAIR: Keith Loftin, Chemist, U.S. Geological Survey
Michael A. Quilliam, The National Research Council of Canada
Certified Reference Materials for Cyanotoxins
Sandra M.F.O. Azevedo, Federal University of Rio de Janeiro
Cyanotoxins Occurrence in South America: Analytical Limitations and Main Challenges
Linda Lawton, Robert Gordon University
Cyanotoxin Occurrence and Analytical Limitations in Africa
Keith Loftin, U.S. Geological Survey
Issues and Proposed Criteria for Validation of Analytical Methodology for Cyanotoxin Detections
Oral Poster Presentation:
Armah de La Cruz, U.S. EPA
A Pilot Study to Evaluate Roof-Collected Rain Barrel Water Quality
Roundtable on Gluten Detection: Achievements or Else?
The new Codex revised standard for gluten labeling includes “very low gluten” in addition to “gluten-free”. From the analytical point of view the two cut-off values and the narrow difference that separate them, 80 ppm (20 and 100 ppm respectively), may impact the labeling strategy in management decisions given the complexity of gluten proteins and the analytical challenges. These challenges are driven by the diversity of analytical results provided by different ELISA kits, the complexity of process food samples, the use of new extraction procedures and the lack of reference materials for validation purposes. Consequently, are we providing consumer with the accurate statements they need to make informed food choices? This roundtable will bring regulatory, industry and kit manufacturers to the table to discussed the pros and cons of new regulatory approaches as well as the implications of analytical results. This session may serve as a crystal ball to evaluate potential consequences of threshold values on allergen detection.
CO-CHAIR: Carmen Diaz-Amigo, Director of Conference Management, Eurofins-CTC
CO-CHAIR: Bert Popping, Director, Molecular Biology and Immunology, Eurofins Scientific Group
Samuel Benrejeb Godefroy, Health Canada
Franz Ulberth, European Commission - Institute for Reference Materials and Measurements
James Norman Roberts, Australian Government - National Measurement Institute Australia
Prasad Rallabhandi, U.S. FDA - CFSAN
Sigrid Haas-Lauterbach, R-Biopharm AG
Mohamed Abouzied, Neogen Corporation
Bruce W. Ritter, ELISA Technologies, Inc.
Masahiro Shoji, Morinaga Institute of Biological Science, Inc.
Elisabeth Halbmayr, Romer Labs Division Holding GmbH
Tony Treloar, ELISA Systems
Jupiter M. Yeung, Nestlé Gerber
11:45 am - 3:00pm
No Scientific Sessions. Please use this time to visit the Exhibit Hall.
3:00 pm - 4:30 pm
Roundtable on Pesticide Formulations: Analytical Challenges, Regulatory Issues, Plus Unique Analyses
Roundtable discussions open to all attendees on issues important to all Pesticide and Disinfectant Product Chemistry analysts. The speakers are from government and industry and will share their views on current challenges in the pesticide and disinfectant enforcement arena. A representative from U.S. EPA Registration Division will speak on current registration standards regarding analytical enforcement methods. Attendees are encouraged to add their experiences to the discussion.
CO-CHAIR: Adrian W. Burns, Analytical Chemist, Active Ingredients Program Coordinator, U.S. EPA
CO-CHAIR: Diane M. Rains, Analytical Chemist, U.S. EPA
Walter K. Gavlick, Monsanto Company
Oliver O. Bennett, Jr. , Kansas Department of Agriculture
Rose Mary Nelson, Dow AgroSciences, LLC
Marian F. Weber, NuFarm Americas, Inc.
Kenneth James Welch, S.C. Johnson & Son, Inc.
Shyam Mathur, U.S. EPA - OPP
TDRM Symposium: Use of Reference Materials and Resources
Reference materials (RMs) can (should) be incorporated in all phases of analytical chemistry: from method development and validation to determination of method accuracy in routine use. In this session, we will cover what makes a good RM and the selection and use of RMs in these various situations. AOAC INTERNATIONAL’s Technical Division on Reference Materials (TDRM) provides a number of resources to the reference material users community, including a pamphlet on the production and use of in-house RMs and a database of RMs organized by analyte, matrix, and Official Method, which will also be reviewed.
CHAIR: Katherine E. Sharpless, Research Chemist, NIST
Mitzi Rettinger, Cerilliant Corporation
What Makes a Good Reference Material or Spiking Solution?
Reinhard Zeleny, European Commission - Institute for Reference Materials and Measurements
Use of RMs in Method Development and Validation
James Neal-Kababick, Flora Research Laboratories
Use of RMs for Establishing Accuracy of Methods in Routine Use
Donna L. Zink, AIM Research Enterprises
TDRM Database of RMs
Oral Poster Presentation:
Katherine E. Sharpless, NIST
Status of NIST Activities to Produce Standard Reference Materials for Dietary Supplements
Natural Toxins: Impact and Methods Needs in the Developing World
This session focuses on current needs and prospective analytical strategies to combat the occurrence of natural toxins in food, with a focus on the developing world. Reliable analytical methods for known toxins as well as modern screening methods for toxins of emerging interest are needed while facing limited resources. The perspective of changing toxin occurrence pattern in the food chain, is an additional challenge to the analytical community worldwide. Therefore straight forward and robust methods are needed and especially in the developing world as well as modern techniques that allow rapid screening for new potential toxins. This demand has also gained interest in view of global indicators for climate changes and expected changes in natural toxin occurrence in both the terrestrial and marine environments. This session will put a focus on the current needs, expected future considerations for testing strategies and, where applicable, on training efforts.
CO-CHAIR: James M. Hungerford, Research Chemist, U.S. FDA - ORA, ATC
CO-CHAIR: Vera Trainer, Supervisory Research Oceanographer, U.S. NOAA - Fisheries, Marine Biotoxin Group
Gordon Seymour Shephard, South African Medical Research Council
Mycotoxins in Developing Countries: Impact and Analytical Needs
Benjamin A. Suarez-Isla, University of Chile
Transfer of Receptor Binding Assays for HABs Toxins: An IAEA-Supported Partnership to Mitigate Public
Health and Socio-Economic Impacts in U.N. Member States
Darsa P. Siantar, U.S. Department of Treasury - TTB
Detection, and Rapid Resolution Liquid Chromatography Tandem Mass Spectroscopy Method Development
Vera Trainer, U.S. NOAA - Fisheries, Marine Biotoxin Group
North Pacific Marine Science Organization (PICES) Harmful Algal Bloom International Seafood Safety Project
Oral Poster Presentation:
Elisabeth Halbmayr, Romer Labs Division Holding GmbH
New Reliable Methods and Reference Materials for Ergot Alkaloids and Pyrrolizidin Alkaloids
Wednesday, September 29, 2010
8:15 am - 9:45 am
New Blood: Developing Methods for the Detection of Chemical Contaminants
The detection of chemical contaminants in food and drugs is an interesting and continually evolving discipline. In order to maintain a vibrant and active community, young scientists must be encouraged to work in this area. The purpose of this session is to give the new members of our community an opportunity to present their work.
CO-CHAIR: Michael A. McLaughlin, Director Regulatory Scientist, U.S. FDA - ORA/DFS
CO-CHAIR: Alexander J. Krynitsky, Chief, Bioanalytical Methods Branch, U.S. FDA - CFSAN
Shaun MacMahon, U.S FDA - CFSAN
Detection of Compounds with High Nitrogen Content Used in Protein Adulteration by LC-MS/MS
Yang Shi, Thermo Fisher Scientific
Advances in Automation of Food-Related Analysis
Kai Zhang, FDA - CFSAN
Determination of Pesticides Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS), Information Dependent Acquisition Triggered Enhanced Product Ion (IDA-EPI) Spectrum and Full Library Search
Sara Edison, U.S. FDA - Forensic Chemistry Center
Rapid Screening for Pesticides Using Ambient Pressure Desorption Ionization (with) High Resolution Mass Spectrometry
Oral Poster Presentation:
André Schreiber, Applied Biosystems
Authenticity Testing of Liquor Samples Using LC-MS/MS Analysis and Statistical Data Analysis
Hot Topic Symposium: Food Fraud and Food Authenticity: How to Ensure to Get Your Money's Worth
Food fraud has a long history. The first book on this topic was written by Frederick Accum in 1820 with the title "A Treatise on Adulterations of Food, and Culinary Poison". Reviewers of the book at the time wrote: "Our Pickles are made green by copper; our vinegar rendered sharp by sulphuric acid…". Food fraud has come a long way since then and became much more sophisticated, much more difficult to detect. The cost of this fraud to global food industry is estimated by some experts to reach $49 billion. Current examples of food fraud include the well-known melamine story, but many, many more issues: food supplements including cod liver oil, aloe vera, mozzarella and coffee have been found to be adulterated; lesser quality wines have been sold as expensive ones, the sale of Arabica /Robusta coffee bean mixes as pure superior Arabica quality, and countless others. This session will deal with approaches by industry and governments to detect the more and more sophisticated adulteration strategies and prevent fraud. Methods and insights will be presented at this Hot Topic Session.
CHAIR: Bert Popping, Director, Molecular Biology and Immunology, Eurofins Scientific Group
Sophie Rollinson, Food Standards Agency
Food Authenticity in the UK: The Challenges, the Successes and New Developments
Martin Stutsman, U.S. FDA - CFSAN
Pomegranate Juice Authenticity Issues and Methodology
Jana Hajslova, Institute of Chemical Technology, Prague
Meat Freshness Authentication Based on Metabolomic Profiling
Robert Fotheringham, Glen Keith Distillery
Brand Protection: Authentication of Spirits
8:15 am – 11:45am
Chemical Authentication of Botanicals Using Chromatographic and Spectral Fingerprinting Methods
Chemical fingerprinting, in combination with chemometrics, specifically pattern recognition methods, has become a powerful tool for identification, authentication, and quality assurance of botanical materials and herbal medicines. In the last 10 years, the World Health Organization (WHO), the European Agency for Evaluation of Medicinal Products (EMEA), the U.S. Food and Drug Administration (FDA), and Chinese State Food and Drug Administration (SFDA) have endorsed chromatographic fingerprinting as a means of authentication and/or monitoring the stability of traditional herbal medicines. This methodology is especially useful when the active components of the botanical material are unknown. The basic philosophy is to use the chemical entirety of the botanical as a signature, rather than one or a few chemical markers. Thus, the focus will be on the use of chemical fingerprints for the identification and authentication of botanical materials and not the analysis of individual components. This symposium will present invited talks by experts on chromatographic and spectral fingerprinting, chemometrics, and the development of SLVs and full validation of identification methods.
CO-CHAIR: James M. Harnly, Research Leader, USDA - ARS BHNRC
CO-CHAIR: Mark C. Roman, President, Tampa Bay Analytical Research, Inc.
Yi-zeng Liang, Central South University - Research Center of Modernization of TCM
Chromatographic Fingerprinting Technique Combined with Chemometrics for Quality Control of TCM
Young Hae Choi, Leiden University
Application of NMR-Based Metabolomics to Chemical Classification of Plants
Peter de Boves Harrington, Ohio University Center for Intelligent Chemical Instruments
Chemometric Strategies for Authentication of Foods and Herbal Supplements
James N. Harnly, - USDA ARS BHNRC
Identification of Panax Species Using MS and UV Fingerprints
Mark C. Roman, Tampa Bay Analytical Research, Inc.
Validation of Qualitative Methods for Botanicals
Oral Poster Presentation:
Zoe Grosser, PerkinElmer, Inc.
The Analysis of Traditional Chinese Herbal Medicines and Supplements for Metals
10:15 am - 11:45 am
I Want to Use a New Microbiology Method: How Do I Validate Methods for Use in My Own Lab?
Method certification through AOAC-RI and validation through AOAC-OMA demonstrates a method meets defined criteria. This however, does not guarantee that every AOAC method will "work" for your particular products and processes. Each lab should conduct a validation of the methods they choose to use, to determine if they are "fit for the purpose" in which they will used. Sound good - but how does one go about doing this?! Building on the 2009 AOAC symposium: Microbiological Method Verification Workshop, REAL-LIFE examples will be outlined and discussed in this symposium, to help bring deeper understanding and practical use to those who are responsible for method determination and implementation within their companies.
CO-CHAIR: Michelle Iannucci, Associate Principal Scientist, Kraft Foods, USA
CO-CHAIR: Pamela Wilger, Global Manager f Food Safety Training, Cargill
Karen M. Silbernagel, AOAC Research Institute
How to Determine if a Microbiology Rapid Method is Right for Your Lab
Pamela Wilger, Cargill
Example of a Quantitative Validation: Real Life Example Presented from Industry
Michelle Iannucci, Kraft Foods, USA
Example of a Qualitative Validation: Real Life Example Presented from Industry
Erin Sutphin Crowley, Q Laboratories, Inc.
The Third Party Independent Laboratory Perspective: Validation of Multiple Microbiological Methods
TDLM Symposium: Why Did That Happen? And How Do I Fix It? Root Cause Analysis and CAPA as Told by Those Who Live It Every Day in the Lab
“The procedure for corrective action shall start with an investigation to determine the root cause(s) of the problem.” The requirement for Corrective Action in ISO/IEC 17025:2005 can be difficult for labs to meet; however, it is important for good business, for efficiency and for compliance. The goal of this workshop is to take the difficulty and pain out of root cause analysis. Hear from a laboratory expert the principles and techniques used in Root Cause Analysis. Then work through real life examples in a workshop format to practice using the effective methods presented. You will gain an understanding on how to approach and document root cause investigations. The examples will be taken from actual cases and presented by people who deal with root cause analyses in the lab, people who have “lived the pain”. ISO/IEC 17025 also requires Monitoring of Corrective Actions. “The laboratory shall monitor the results to ensure that the corrective actions taken have been effective.” When you find a root cause you want to eliminate it to ensure costly issues are dealt with quickly and effectively. No one enjoys fixing the same nonconformance over and over. Learn how you can determine and prove that the Corrective Action worked and know the cause of that nonconformance has been eliminated. After the presentations, real life examples will be presented for you to work through, either with a facilitator or on your own. There will be limited seats at the tables where the facilitators will help people work through the real life examples, so arrive early to ensure you get a seat at a table with a facilitator.
CHAIR: Michele Smoot, Corporate Director of Microbiology, Silliker, Inc.
Cathy L. Burns, U.S. FDA – DHS
The Corrective Action Process
Kenneth P. Stoub, Group Seven Laboratory Services
Root Cause Analysis and Corrective Action
1:00 pm - 2:30 pm
New Validation Strategies for the 21st Century
A look at some new and exciting validation ideas from around the world, with a special focus on validation of microbiological methods.
CO-CHAIR: Paul Wehling, Senior Scientist, General Mills, Inc.
CO-CHAIR: Max H. Feinberg, INRA
Max H. Feinberg, INRA
Accuracy Profile as a Tool for Interpreting Microbiological Validation Data in ISO 16140
Paul Wehling, General Mills, Inc.
Probability of Detection (POD) Model for the Validation of Qualitative Methods of Analysis
Sharon L. Brunelle, AOAC Research Institute
Implementation of the POD Model in AOAC Microbiological Validation Studies
Paul H. In't Veld, Food and Consumer Product Safety Authority
Revision if ISO 16140: Progress and Harmonization with AOAC
Disinfectants: Exotica Redux
Consumer and industrial products claiming to be sanitizers, disinfectants, and liquid chemical sterilants must be registered with the US EPA before they can be sold as antimicrobials. Some scientifically important agents are difficult to cultivate or cannot be cultured in the laboratory; thereby making registration problematic. This is equally true with device-associated disinfectants and biopharmaceuticals regulated by the US FDA. This symposium looks at problematic microorganisms capable of causing problems throughout the world.
CHAIR: Donna B. Suchmann, MicroBioTest, Division of Microbac Laboratories
Joseph R. Rubino, Reckitt Benckiser, Inc
The Next Pandemic: What Can We Expect?
Alice A. Young, Naval Surface Warfare Centre
A Comparison of Bacillus anthracis Spore Decontamination Using the AOAC 2008.05 Three-Step Method and a Single Spore Extraction Method
Steve Zhou, MicroBioTest, Division of Microbac Laboratories
Virus and Transmissible Spongiform Encephalopathy Agent-Contamination of Biologics: Testing and Process Evaluation
Harnessing Information: Digital and Analog Approaches to Pathogen Detection
The globalization of food production has provided consumers with an astonishing variety of fresh and processed foods. An unfortunate consequence of such diversity is the possible exposure to geographically and microbiologically diverse foodborne pathogens and toxins. The challenge to researchers is to be able to rapidly identify the strain of the microorganism or the toxin or prion responsible for an outbreak. Recent advances in sequencing technology permit the rapid determination of bacterial genomes. Bacterial strains can be distinguished by their characteristic gene sequences. This genomic data can be used to generate the PCR primers necessary to yield amplicons characteristic of specific bacterial strains. Mass spectrometry can be used to identify these amplicons by their distinct molecular weight, thereby permitting the rapid analysis of complex mixtures of molecules. Bacterial toxins persist long after the producing bacterium has died and its DNA has been degraded or they can diffuse or be carried far from the producing organism. In either case, a DNA-based detection system would not be able to detect these toxins. Advances in robotics, molecular biology, and molecular immunology have dramatically improved antibody production. The antibodies produced by these techniques are being used to develop sensitive assays for these toxins. Prions are novel pathogens that do not require nucleic acids to replicate. They are able to recruit a normal cellular protein and convert it into a prion and thereby propagate an infection. The information necessary to propagate a prion is enciphered in its conformation. Mass spectrometry can be used to detect and quantitate prions based on the physico-chemical characteristics of the prion isoform. Both the digital (DNA-based) and the analog (protein-based) approaches are essential to characterizing these bacterial strains, toxins, and prions.
CHAIR: Christopher J. Silva, Research Chemist, USDA - ARS
Steven A. Hofstadler, Ibis Biosciences, Inc.
Detection and High Resolution Strain Typing of Microorganisms with PCR and High Throughput Mass
Spectrometry: Platform Fundamentals and Applications
William G. Miller, USDA, ARS, WRRC
Comparative Genomics of the Genus Campylobacter: Evolution, Environmental Adaptation, Host Association and Pathogenicity
Christopher J. Silva, USDA - ARS
Mass Spectrometric Approaches to Detecting Conformational Differences in Proteins
Miles Scotcher, USDA - ARS
Antibodies in the Detection of Foodborne Contaminants: New Tricks for Old Dogs!"
Oral Poster Presentation:
Olga Petrauskene, Applied Biosystems, Inc.
Novel Efficient Sample Preparation Combined with Lyophilized Real-Time PCR Assays for Highly Sensitive and Accurate Detection of Foodborne Pathogens by Real-Time PCR from Complex Food Matrices
3:00 pm - 4:30 pm
Analytical Challenges: Mycotoxins and Food Safety
Despite having well established validated methods for routine regulatory purposes (official methods), there still remain analytical challenges in obtaining reliable data on human exposure and in demonstrating the effectiveness of prevention and control strategies. This session will cover the challenges in measuring ‘masked mycotoxins’ and their significance in human exposure. Human biomarkers in urine or plasma need to be accurately determined if they are to be used to assess exposure, and baseline levels of mycotoxins in food need to be determined prior to introduction of prevention and control strategies. Additionally the effectiveness of intervention strategies such as use of chemisorbents in animal feed and in human studies can only be demonstrated with good analytical tools.
CO-CHAIR: John Gilbert, Director, FoodLife Int. Ltd
CO-CHAIR: Michal Godula, Food Safety Specialist, Thermo Fisher Scientific
Jana Hajslova, Institute of Chemical Technology, Prague
Analytical Challenges in Determining "Masked" Mycotoxins in Foodstuffs
Gordon Seymour Shephard, South African Medical Research Council
Analytical and Other Challenges in Measuring Human Mycotoxin Biomarkers
Timothy D. Phillips, Texas A&M University
Analytical Challenges in Using Clay-Based Enterosorbents for Mycotoxins in Human and Animal Studies
Angelo Visconti, CNR-ISPA, Institute of Sciences of Food Production
Analytical Challenges in Developing Prevention and Control Strategies in Food and Feed
Phytochemical Analysis of Herbal Raw Materials and Regulatory Challenges
In the dietary supplement industry today, raw materials are procured from some part of the world and manufactured in the other while marketed somewhere else. Due to lack of harmonization, different markets and countries have different regulatory requirements leading to a very challenging situation for the acceptability of “generic” products worldwide. Also, today’s consumer demands supplements that have proven health benefits. This often leads to formulations that may contain active pharmaceutical ingredients. This symposium is dedicated to share examples and discussion on three important aspects surrounding the quality of dietary supplements. Those are botanical authenticity and benchmarking for total quality control, general statutory and regulatory requirements for safety and labeling of new dietary ingredients as well as their forensic analysis for detecting active pharmaceuticals. Speakers include scientific experts from dietary supplement manufacturers and FDA.
CO-CHAIR: Amit Chandra, Research Associate, Analytical Services, AMWAY
CO-CHAIR: Jat Rana, Research Associate, Analytical Services, AMWAY
Amit Chandra, AMWAY
The Power of Fingerprints
Robert J. Moore, U.S. FDA - CFSAN
General Regulatory Requirements for Marketing Dietary Supplements and Dietary Ingredients
Doug T. Heitkemper, U.S. FDA - ORA
Forensic Analysis of Dietary Supplements for Active Pharmaceutical Ingredients
Oral Poster Presentation:
Heidi M. Evenocheck, Access Business Group
Chitosan and Chitin Analysis by HPLC
XRF for Screening of Foods and Nutritional Supplements; Capabilities, Use and Limitations
The Symposia will cover uses of XRF (ED,WD and T XRF) from lab to field with respect to inorganic element determination (contaminants) in food and dietary supplements. The Symposia is aimed to provide increase the knowledge of the XRF technology when applied to the foods, nutritional supplement and nutraceutical segment.
CHAIR: Alexander Seyfarth, Bruker AXS Inc.
Michael Rider, Bruker AXS Inc.
Traditional ED XRF for Screening of Foods, Nutritional Supplements and Imports: Capabilities, Use and
Limitations
Peter T. Palmer, San Francisco State University
Rapid Differentiation of Authentic and Counterfeit Pharmaceutical Products via XRF
Paula N. Brown, British Columbia Institute of Technology
Elemental Fingerprinting of Panax quinquefolius by Total Reflection X-Ray Fluorescence (TXRF) with
Multivariate Data Analysis to Establish Provenance
Lori Paley, B.S. , British Columbia Institute of Technology
Total Reflection X-ray Fluorescence (TXRF) with Multivariate Data Analysis as a Tool to Differentiate
Geographic Origins of Okanagan Merlot Grapes
