METHODS NEWS

Single-Laboratory Validation in Process

Single-Laboratory Validation Approved

Performance Tested Method^SM Submitted for Review

Performance Tested Methods^SM Approved

Precollaborative Study Protocols Submitted for Review

Precollaborative Study Protocols Approved

Precollaborative Study Manuscripts Submitted for Review

Precollaborative Study Manuscripts Approved

Collaborative Study Protocols Submitted for Review

Collaborative Study Protocols Approved

Collaborative Study Manuscripts Submitted for Review

Method Modifications Submitted for Review

Method Modifications Approved

Methods for Possible First Action Official Methods^SM Adoption

Methods Adopted as First Action Official Methods^SM

Methods Approved Final Action

Methods Approved for Repeal

The AOAC^® Official Methods Program^SM is designed to provide fully validated methods that can be used with confidence by regulatory agencies, regulated industry, product testing laboratories, and academic institutions. They are subjected to an eight or more laboratory collaborative study according to internationally recognized standards and receive rigorous scientific review of performance results. Adoption of a method is based on the demonstration of its reliability and practicality by completion of a successful collaborative study.

Notice of official actions and changes in the status of methods currently in review are listed below.

Any interested party may submit comments and data in writing for or against methods recommended for Repeal, or Final Action. To comment, or for information on how to submit a method, or become a collaborator, General Referee, Study Director, Method Advisor, or Topic Advisor, contact Deborah McKenzie, AOAC INTERNATIONAL, 481 N. Frederick Ave, Suite 500, Gaithersburg, MD 20877-2417, USA, Tel: +1-301-924-7077 ext. 157, Fax: +1-301-924-7089, e-mail: dmckenzie@aoac.org

Single-Laboratory Validation in Process

[Data collected from internal/independent laboratory and accepted by the General Referee, Statistician, Safety Advisor, and Methods Committeeunder review by Chief Scientific Officer and experts (consensus-based criteria)]

Lutein and Zeaxanthin in Supplements and Raw Materials by Liquid Chromatography
Contact Tatania K. Emmick, Kemin Health, 2100 Maury St, Des Moines, IA 50301, USA, Tel: +1-515-875-4461, Fax: +1-515-559-5232, e-mail: tatania.emmick@kemin.com

Alpha, Beta, Gamma, and Delta Tocopherol; Alpha, Beta, Gamma, and Delta Tocotrienol; and Alpha Tocopherol Acetate in Dietary Supplements by High-Performance Liquid Chromatography
Contact John Austad, Covance Laboratories Inc., 3301 Kinsman Blvd, Madison, WI 53704, USA, Tel: +1-608-242-2712, Fax: +1-608-242-7903, e-mail: john.austad@covance.com

DHA, EPA, and ALA Omega-3 Fatty Acids in Dietary Supplements by Gas Chromatography with Flame Ionization Detection
Contact Sandy Diltz, Martek Biosciences Corp., 6480 Dobbin Rd, Columbia, MD 21045, USA, Tel: +1-443-542-2186, Fax: +1-410-740-2985, e-mail: sdiltz@martekbio.com

Single-Laboratory Validation Approved

[Data collected from internal/independent laboratory and approved by Chief Scientific Officer and experts (consensus-based criteria)]

Precollaborative Study Protocols Submitted for Review

[Proposed outlines for in-house studies (typically microbiological methods only) under review by the General Referee, Safety Advisor, Statistician, and Methods Committee]

Precollaborative Study Protocols Approved

[Proposed outlines for in-house studies (typically microbiological methods only) accepted by the General Referee, Safety Advisor, Statistician, and Methods Committee]

Enterobacter sakazakii in Powdered Infant Formula by the Assurance for Enterobacter sakazakii, OMA-2007-Apr-007
Contact Phil Feldsine, BioControl Systems, Inc., 12822 SE 32nd St, Bellevue, WA 98040, USA, Tel: +1-425-603-1123, Fax: +1-435-586-3308, e-mail: ptf@biocontrolsys.com

Enterobacter sakazakii in Powdered Infant Formula by the BAX ® Assay for the Detection of Enterobacter sakazakii, OMA-2007-Apr-010
Contact Morgan Wallace, Dupont Qualicon, ESL Bldg 400, Rt 141 and Henry Clay Rd, Wilmington, DE 19880, USA, Tel: +1-302-695-5473, Fax: +1-302-695-5877, e-mail: morgan.wallace@usa.dupont.com

Evaluation of the Botulinum Toxin ELISA for the Detection of Toxins A, B, E, and F in Select Foods, OMA-2007-Oct-037
Contact Richard Whiting, U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, HFS-300, 5100 Paint Branch Pkwy, College Park, MD 20740-3835, USA, Tel: +1-301-436-1925, Fax: +1-301-436-2632, e-mail: richard.whiting@fda.hhs.gov

Precollaborative Study Manuscripts Submitted for Review

[Data collected from completed in-house studies (typically microbiological methods only) under review by the General Referee, Safety Advisor, Statistician, and Methods Committee]

Enterobacter sakazakii (Cronobacter) in Powdered Infant Formulae with the foodproof Enterobacteriaceae plus E. sakazakii Detection System, OMA-2009-Mar-004
Contact Benjamin Diep, Nestlé Research Center, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland, Tel: +41 21 785 8652, Fax: +41 21 785 8553, e-mail: benjamin.diep@rdls.nestle.com

Precollaborative Study Manuscripts Approved

[Data collected from completed in-house studies (typically microbiological methods only) accepted by the General Referee, Safety Advisor, Statistician, and Methods Committee]

Evaluation of a Revised Bacteriological Analytical Manual Method for the Detection of Enterobacter sakazakii in Powdered Infant Formula, OMA-2008-Jul-026
Contact Keith Lampel, U.S. Food and Drug Administration, Office of Regulatory Science, 5100 Paint Branch Pkwy, College Park, MD 20740, USA, Tel: +1-301-436-2007, e-mail: keith.lampel@fda.hhs.gov

Collaborative Study Protocols Submitted for Review

[Proposed protocol for study designs involving 8 (quantitative) to 10 (qualitative) independent laboratories under review by the General Referee, Safety Advisor, Statistician, and Methods Committee. Please contact the primary author if your laboratory is interested in participating in the collaborative study.]

Collaborative Study Protocols Approved

[Data collected from 8 (quantitative) and 10 (qualitative) independent laboratories accepted by the General Referee, Statistician, Safety Advisor, and Methods Committee]

Bovine Immunoglobulin G in Bovine Colostrum, Bovine Milk, and Bovine Dietary Supplements by Protein G Affinity Liquid Chromatography
Contact Grant Abernethy, Fonterra Cooperative Group Ltd., Dairy Farm Rd, Private Bag 11029, Palmerston North, New Zealand, Tel: +64 6 350 4649 ext. 66303, Fax: +64 6 350 1467, e-mail: Grant.Abernethy@fonterra.com

Department of Defense Critical Reagents Program and National Guard Bureau Detection of Virulent and Avirulent Bacillus anthracis Spores From Dry Filter Unit Filters by PCR
Contact Diane Dutt, Noblis, Aberdeen Proving Ground, Edgewood Area, BioDefense ASMRD-ECB-RT-BD, Bldg E-3150, N. Kingscreek Rd, Aberdeen Proving Ground, MD 21010, USA, Tel: +1-410-436-7831, e-mail: diane.dutt1@us.army.mil

National Guard Bureau Detection of Virulent and Avirulent Bacillus anthracis Spores by PCR
Contact Diane Dutt, Noblis, Aberdeen Proving Ground, Edgewood Area, BioDefense ASMRD-ECB-RT-BD, Bldg E-3150, N. Kingscreek Rd, Aberdeen Proving Ground, MD 21010, USA, Tel: +1-410-436-7831, e-mail: diane.dutt1@us.army.mil

Immunoglobulin G in Bovine Colostrum, Milk Products, and Dietary Supplements by Surface Plasmon Resonance
Contact Leyton Gapper, Fonterra Cooperative Group Ltd., Dairy Farm Rd, Private Bag 11029, Palmerston North, New Zealand, Tel: 64 6 350 4600 ext. 7503, Fax: 64 6 350 4607, e-mail: leyton.gapper@fonterra.com

Oxytetracycline in Animal Feed, Fish Feed, and Animal Remedies, Liquid Chromatrography with Fluorescence Detection, OMA-2004-Oct-017
Contact Nancy Thiex, South Dakota State University, Olson Biochemistry Laboratories, Box 2170 ASC 36A, Brookings, SD 57007-1217, USA, Tel: +1-605-688-5466, Fax: +1-605-688-6295, e-mail: nancy.thiex@sdstate.edu

Chromatography with Fluorescence Detection, OMA-2004-Oct-017
Contact Nancy Thiex, South Dakota State University, Olson Biochemistry Laboratories, Box 2170 ASC 136A, Brookings, SD 57007-1217, USA, Tel: +1-605-688-5466, Fax: +1-605-688-6295, e-mail: nancy.thiex@sdstate.edu

Bifenthrin Analysis in Technical Materials and Formulations by Gas Chromatography, OMA-2005-May-012
Contact Ed Kikta, FMC Corp., R&D, PO Box 8, Princeton, NJ 08543-008, USA, Tel: +1-609-951-3640, Fax: +1-609-951-3835, e-mail: edward_kikta@fmc.com

966.04 Preliminary Evaluation of Cooked Meat Medium with Manganese Sulfate for the Cultivation of Clostridium sporogenes, OMA-2006-Nov-075
Contact Stephen Tomasino, U.S. Environmental Protection Agency, Environmental Science Center, 701 Mapes Rd, Fort Meade, MD 20755-5350, USA, Tel: +1-410-305-2976, Fax: +1-410-305-3094, e-mail: Tomasino.Stephen@epa.gov

Field Study of National Guard Bureau Swab Environmental Sampling Method, OMA-2007-Jul-026
Contact Diane Dutt, 5183 Blackhawk Rd, Aberdeen Proving Ground, Aberdeen, MD 21010, USA, Tel: +1-410-436-7831, e-mail: diane.dutt1@us.army.mil

TEMPO® EC for the Enumeration of Escherichia coli in a Variety of Foods and TEMPO® CC for the Enumeration of Total Coliform Counts in a Variety of Foods, OMA-2007-Nov-041 Contact Ronald Johnson, bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 65042, USA, Tel: +1-314-506-8182, Fax: +1-314-731-8276, e-mail: ron.johnson@na.biomerieux.com

Methylsulfonylmethane (MSM) Content in Raw Materials and Dietary Supplements by GC-FID, OMA-2007-Nov-046.R1
Contact Mark Roman, Tampa Bay Analytical Research, Inc., PO Box 931, Safety Harbor, FL 34695, USA, Tel: +1-727-422-8028, Fax: +1-484-910-8727, e-mail: mroman@tampabayanalytical.com/p>

Dimethylsulfoxide (DMSO) in Methylsulfonylmethane (MSM) Raw Materials by GC-FID, OMA-2007-Nov-048.R1
Contact Mark Roman, Tampa Bay Analytical Research, Inc., PO Box 931, Safety Harbor, FL 34695, USA, Tel: +1-727-422-8028, Fax: +1-484-910-8727, e-mail: mroman@tampabayanalytical.com

Ginsenosides in Panax ginseng and Panax quinquefolius Raw Materials and Finished Products by High-Performance Liquid Chromatography, OMA-2008-Feb-005
Contact Paula Brown, British Columbia Institute of Technology, Herbal Evaluation and Analysis Laboratory, 3700 Willingdon Ave, Burnaby, British Columbia V5G 3H2, Canada, Tel: 604 412 7484, Fax: 604 433 5893, e-mail: Pbrown@bcit.ca

VIDAS Listeria Species Xpress (LSX) for the Detection of Listeria Species in Foods, OMA-2008-Feb-007
Contact Ron Johnson, bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 65042, USA, Tel: +1-314-506-8182, Fax: +1-314-731-8276, e-mail: ron.johnson@na.biomerieux.com

Paralytic Shellfish Poisoning in Shellfish by Receptor Binding Assay, OMA-2008-Mar-016
Contact Frances van Dolah, National Oceanic Atmospheric Administration/NOS Center for Coastal Environmental Health and Biomolecular Research, Charleston, SC, USA, Tel: +1-843-762-8529, e-mail: fran.vandolah@noaa.gov

S-Adenosyl-L-Methionine in Dietary Supplements and Raw Materials by Reversed-Phase High-Performance Liquid Chromatography with UV Detection, OMA-2008-May-019
Contact Wendy Sorenson, Covance Laboratories, Inc., 3301 Kinsman Blvd, Madison, WI 53704, USA, Tel: +1-608-310-8206, Fax: +1-608-310-8200, e-mail: wendy.sorenson@covance.com

Total Carbohydrates in Wine and Wine-Like Beverages by HPLC, R1, OMA-2008-Sep-029
Contact Steve Kupina, Constellation Wines, US, 12667 Rd 24, Madera, CA 93637, USA, Tel: +1-559-661-5548, e-mail: steve.kupina@cwine.com

Detection of Salmonella species in Selected Foods by the Assurance GDSTM for Salmonella, OMA-2008-Sep-031
Contact Philip Feldsine, BioControl Systems Inc., 12822 SE 32nd St, Bellevue, WA 98005-4318, USA, Tel: +1-425-603-1123, Fax: +1-425-603-0070, e-mail: ptf@biocontrolsys.com

Collaborative Study Manuscripts Submitted for Review

[Final study manuscripts detailing results, data analysis, and conclusions from 8 (quantitative) to 10 (qualitative) independent laboratories under review by the General Referee, Statistician, Safety Advisor, and Methods Committee]

Gliadin as a Measure of Gluten in Foods, OMA-2008-Jan-004
Contact Ulrike Immer, R-Biopharm AG, Landwehrstr. 11, Darmstadt 64293, Germany, Tel: 0049 6151 8102 38, Fax: 0049 6151 8102 20, e-mail: u.immer@r-biopharm.de

Chondroitin Sulfate Content in Raw Materials and Dietary Supplements by HPLC-UV After Enzymatic Hydrolysis, OMA-2008-Feb-010
Contact Mark Roman, Tampa Bay Analytical Research, Inc., PO Box 931, Safety Harbor, FL 34695, USA, Tel: +1-727-422-8028, Fax: +1-484-910-8727, e-mail: mroman@tampabayanalytical

Lycopene in Dietary Supplements and Raw Materials by High-Performance Liquid Chromatography, OMA-2008-Mar-017
Contact Jayant Deshpande, OmniActive Health Technologies Pvt. Ltd, A-131, Wagle Industrial Estate, Thane, India 400604, e-mail: j.deshpande@omniactives.com or Ed Waysek, Caravan Ingredients Inc., 100 Adams Dr, Totowa, NJ 07512, USA, Tel: +1-973-256-8886, Fax: +1-973-256-8025, e-mail: ewaysek@caravanproducts.com

Vitamin B12 in Fortified Bovine Milk-Based Infant Formula, Fortified Soya-Based Infant Formula, Fortified Baby Food, Fortified Cereals, Vitamin Premix, and Fortified Dietary Supplements by Surface Plasmon Resonance, OMA-2008-Oct-032
Contact Pathik Vyas, AgriQuality Laboratory Network, Auckland Laboratory, 131 Boundary Rd, Blockhouse Bay, PO Box 41, Auckland, New Zealand, Tel: 64 9 626 8216, Fax: 64 9 626 8282, e-mail: vyasp@agriquality.com or Anthony A. O'Kane, Xenosense Ltd-Titanic Quarter, e-mail: Anthony.OKane@ge.com

Method Modifications Submitted for Review

[Proposed modifications (procedure changes, editorial changes, analyte additions, method extentions, etc.) of existing AOAC Official Methods under review by the General Referee, Safety Advisor, Statistician, and Methods Committee]

2003.12 Validation of the Equivalence of a Minor Modification to AOAC Method 2003.12 with the Reference Culture Method for the Detection of Listeria monocytogenes in Foods, OMA-2006-Mar-040
Contact Morgan Wallace, Dupont Qualicon, ESL-Bldg 400, Rt 141 and Henry Clay Rd, Wilmington, DE 19880, USA, Tel: +1-302-695-5473, Fax: +1-302-695-5877

Method Modifications Approved

2003.09 Validation of the Equivalence of a Minor Modification to AOAC Method 2003.09 with the Reference Culture Method for the Detection of Salmonella in Foods, OMA-2006-Mar-037
Contact Morgan Wallace, Dupont Qualicon, ESL-Bldg 400, Rt 141 and Henry Clay Rd, Wilmington, DE 19880, USA, Tel: +1-302-695-5473, Fax: +1-302-695-5877

Validation Study to Demonstrate the Equivalence of a Minor Modification of Visual Immunoprecipitate (VIP) for Listeria Method to the Reference Culture Method, OMA-2008-Mar-018 (997.03) Contact Philip Feldsine, BioControl Systems Inc., 12822 SE 32nd St, Bellevue, WA 98005-4318, USA, Tel: +1-425-603-1123, Fax: +1-425-603-0070, e-mail: ptf@biocontrolsys.com

Validation Study to Demonstrate the Equivalence of a Minor Modification of Visual Immunoprecipitate (VIP) for Salmonella Method to the Reference Culture Method, OMA-2008-Jun-022
Contact Philip Feldsine, BioControl Systems Inc., 12822 SE 32nd St, Bellevue, WA 98005-4318, USA, Tel: +1-425-603-1123, Fax: +1-425-603-0070, e-mail: ptf@biocontrolsys.com

Validation Study to Demonstrate the Equivalence of a Minor Modification of Visual Immunoprecipitate (VIP) for E. coli O157:H7 Method to the Reference Culture Method, OMA-2008-Jun-023
Contact Philip Feldsine, BioControl Systems Inc., 12822 SE 32nd St, Bellevue, WA 98005-4318, USA, Tel: +1-425-603-1123, Fax: +1-425-603-0070, e-mail: ptf@biocontrolsys.com

Minor Modification to Official Method^SM 2000.13, Reveal for E. coli O157:H7 Test System in Selected Foods (8-Hour Method): Validation of the Test for 375 g Raw Beef Samles, OMA-2008-Aug-027
Contact Mark Mozola, Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA, Tel: +1-517-372-9200, Fax: +1-517-372-0108, e-mail:mmozola@neogen.com

Minor Modification to Official Method^SM 2000.14, Reveal for E. coli O157:H7 Test in Selected Foods and Environmental Swabs (20-Hour Method): Validation of the Test for 375 g Raw Beef Samples, OMA-2008-Aug-028
Contact Mark Mozola, Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA, Tel: +1-517-372-9200, Fax: +1-517-372-0108, e-mail: mmozola@neogen.com

Improving the AOAC Use-Dilution Method by the Establishment of Minimum and Maximum Log Density Values for Test Microbes on Inoculated Carriers, Method Modification, OMA-2008-Sep-030
Contact Stephen Tomasino, U.S. Environmental Protection Agency, Environmental Science Center, 701 Mapes Rd, Fort Meade, MD 20755-5350, USA, Tel: +1-410-305-2976, Fax: +1-410-305-3094, e-mail: Tomasino.Stephen@epa.gov

A Comparative Evaluation of the VIDAS^® HEAT and GO and the Traditional Boiling Step with VIDAS EasySLM, LSX, and ECO Methods: Modifications to Existing VIDAS Methods 996.06, 999.08, 2001.09, 2004.03, and 2004.06 and Modifications to Existing VIDAS PTM Certifications 981202, 100501, 100702, and 010502, OMA-2008-Nov-033
Contact Ron Johnson, bioMérieux, Inc., 595 Anglum Rd, Hazelwood, MO 65042, USA, Tel: +1-314-506-8182, Fax: +1-314-731-8276, e-mail: ron.johnson@na.biomerieux.com

Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 997.03 Visual Immunoprecipitate (VIP^®) for Listeria to the Reference Culture Method, OMA-2009-Feb-001
Contact Philip Feldsine, BioControl Systems Inc., 12822 SE 32nd St, Bellevue, WA 98005-4318, USA, Tel: +1-425-603-1123, Fax: +1-425-603-0070, e-mail: ptf@biocontrolsys.com

Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 996.09 Visual Immunoprecipitate (VIP^®) for E. coli O157:H7 Method to the Reference Culture Method, OMA-2009-Mar-005
Contact Philip Feldsine, BioControl Systems Inc., 12822 SE 32nd St, Bellevue, WA 98005-4318, USA, Tel: +1-425-603-1123, Fax: +1-425-603-0070, e-mail: ptf@biocontrolsys.com

Methods for Possible First Action Official Methods^SMAdoption

(Methods for possible First Action adoption under review by the General Referee, Safety Advisor, Statistician, and Methods Committee)

Methods Adopted as First Action Official Methods^SM

Methods Approved Final Action

(The following First Action Official Methods^SM were recommended for Final Action status in September 2008 Methods Committee Annual Reports. The Official Methods Board approved a status change for these methods at its January 2009 meeting.)

Methods Approved for Repeal

(The following First Action Official MethodsSM were recommended for Repeal status in September 2008 Methods Committee Annual Reports. The Official Methods Board approved a status change for these methods at its January 2009 meeting.)