Methods for the Quantitation of Collagen

March 6, 2017 [Rockville, Maryland] - AOAC INTERNATIONAL invites method authors and developers to submit relevant methods that may meet the AOAC Standard Method Performance Requirements® (SMPR®) for the Quantitation of Collagen in ingredients and dietary supplements (SMPR 2016.005). Submitted methods will be reviewed by an AOAC Expert Review Panel for consideration of AOAC First Action Official MethodsSM status. Methods adopted as AOAC First Action Official MethodsSM by the Expert Review Panel will be published by AOAC INTERNATIONAL.

The objective of this call for methods is to collect relevant methods that may meet one or more of the aforementioned SMPRs, all of which have been approved by the AOAC Stakeholder Panel on Dietary Supplements (SPDS). All submitted methods will be subjected to evaluation by an AOAC Expert Review Panel, who will review them for potential AOAC First Action Official MethodsSM status. Any resulting approved/adopted Official Methods of Analysis may be used as a reference method. Acceptable methods must demonstrate that they meet one or more of the aforementioned SMPRs, therefore, being reliable and reproducible when used by trained analysts in accredited laboratories.

Methods must be able to identify and quantify individual native (undenatured) and hydrolyzed collagen type I, II, and III if one or multiple types are present in dietary ingredients and dietary supplement finished products. For the purpose of this Call for Methods, collagen is defined as follows:

A triple helix protein that generally consists of two identical chains (α1) and an additional chain that differs slightly in its chemical composition (α2). The amino acid composition of collagen is notable for its particularly high hydroxyproline content. The three most common types of collagen are: type I, found in skin, tendon, vascular ligature, organs, bone (main component of the organic part of bone); type II, found in cartilage (main collagenous component of cartilage); and type III, found in reticular fibers.

Any analytical technique(s) that measures the analytes of interest and meets the method performance requirements in AOAC 2016.005 will be considered.

AOAC invites method authors to submit their methods with no fee required. Interested method authors or developers should provide a copy of their proposed method, as well as any available data characterizing the analytical performance and scientific validity of the method in AOAC format. Interested method authors looking for guidance on formatting methods and manuscripts or other supporting information can find this here. For additional method author resources, please visit the AOAC Author Resource Center.

Ideal method submissions should include a well written method procedure and be accompanied by data or information supporting the method’s applicability and demonstrating the method’s ability to meet the criteria of the AOAC standard method performance requirements.

The copyright transfer form must also be completed for ALL method submissions. If the method has been previously published, permission must be obtained from the appropriate parties. Please provide name(s) and complete contact information for the publishing organization and/or corresponding author.