Online November 19, 2020 | $699 nonmembers – $599 members | Organizational Member discounts

The reliability and significance of results in testing for the presence or absence of food- and water-borne microbial contaminants is dependent not only on the performance and sensitivity of the test method itself, but also on the sampling practices employed. Sampling practices influence the validity and usefulness of all results that stem from any subsequent testing. 

Sampling considerations can be divided into three distinct areas that encompass (i) field-derived samples, (ii) samples derived from manufacturing and processing sites, and (iii) those within the testing laboratory itself. In all three of these scenarios, the goal is to efficiently and confidently capture a sample that best represents the whole.

In collaboration with presenters and subject matter experts from the U.S. Food and Drug Administration (FDA) Center for Food Safety and Applied Nutrition and other nationally recognized experts, this training course will introduce theoretical considerations for the reliable collection of test samples and the practical applications for testing water, soil amendments, fresh produce, and grains for several highly pathogenic microbial contaminants.

Information gained from participation in this training session will provide industry and associated food-safety testing stakeholder communities with important information on how to design effective sampling plans; and how to employ the most relevant methodologies aligned with regulatory practices for obtaining reliable data needed to assess the safety of their products. It is also intended to assist accredited laboratories as they develop sampling plans in compliance with ISO 17025.

Agenda

All times are in EST.

9:00 AM– 9:15 AMWelcome and Introduction, P. Orlandi, AOAC INTERNATIONAL
SESSION 1: FIELD SAMPLINGPart 1:  Water Sampling for Enteric Pathogens:
9:15 AM – 9:40AMDead-end Ultrafiltration (DEUF) for Detection of Shigatoxigenic E. coli in Agricultural Water, J. Kase, FDA
9:40 AM – 10:15AMDEUF for Detection of Cyclospora from Water,
A. DaSilva and M. Durigan
10:15AM – 10:40 AMModified Moore Swab Water Sampling for Salmonella in Agricultural Waters
R. Bell, FDA
Part 2:  Sampling of Other Environmental Matrices of Importance
10:40 AM – 11:05AMSampling of Compost and other Soil Amendments, D. Ingram, FDA
Part 3:  Sampling Field Produce and Grains for Enteric Pathogens
11:05 AM – 11:30 AM Best Practices for Sampling and Processing Field Produce,  D. Macarisin, FDA
11:30 AM – 11:55AMPerspectives on the Sampling of Grain and Grain Products, Sam Myoda, IEH
11:55 AM – 12:15 PMQuestion and Answer Period for the Field Sampling Speakers
12:15 PM – 1:30 PMLunchtime Break
SESSION 2:  SAMPLING IN MANUFACTURING SITES
1:30 PM – 1:55 PMSampling from Large Quantities of Raw Ingredients, G. Sharma, FDA
1:55 PM– 2:20 PMThe Environmental Monitoring Zone Concept and Swabbing, D. Zink, IEH
2:20 PM – 2:40 PMQuestion and Answer Period for the Speakers
SESSION 3:  LABORATORY ANALYSIS OF ENVIRONMENTAL SAMPLES
2:40 PM – 3:05 PMSelective and Non-selective Enrichment Strategies and Non-culturable Microorganisms, T. Hammack, FDA
3:05 PM – 3:30 PMConcentration Techniques: The Role of Immunomagnetic Capture, J. Kase, FDA
3:30 PM – 3:50 PMQuestion and Answer Period for the speakers
SESSION 4:  STATISTICAL CONSIDERATIONS
3:50 PM – 4:20 PMDeveloping a Sampling Plan and Addressing Measurement Uncertainty, B. Buchanan, University of Maryland
4:20 PM – 4:30 PMQuestion and Answer Period
4:30 PM – 4:40 PMClosing Comments and Adjournment