Method Title
Author Listing and Information
Abstract
Introduction
Single Laboratory Validation and/or Multi-laboratory (Reproducibility) Study
Method Document
Results and Discussion (or you may separate these)
Conclusion
Acknowledgments
Conflict of Interest Statement
Funding Disclosure (if applicable)
Supplemental Information (if applicable)
References
Figure Captions
Tables and Figures
Manuscript should be double-spaced, 12 pt, Times New Roman font or any true type font
Sample Paper: https://doi.org/10.1093/jaoacint/qsaa080
Method Title
Validation of the Test Method/Modification for Detection/Determination/Enumeration of Analyte in Matrix(es): First/Final Action 20xx.xx
Author Listing and Information
Abstract
The Abstract must be no more than 300 words in length, contain a brief description of the method, matrixes1 tested and brief summary of results.
1 Note: The term “matrixes” is used in JAOAC publications, instead of matrices.
Abstracts should be organized by background, objective, methods, results, conclusions, highlights with section titles included in bold as shown below:
Background:
Objective:
Methods:
Results:
Conclusions:
Highlights:
Abstract Format
Introduction
The Introduction should supply sufficient background to enable the reader to attain the proper understanding and perspective for the study. Cited references should be listed in sequential numerical order with the reference number indicated in the text with number placed in parentheses (not superscript).
The Introduction should provide the hypothesis that was addressed or the rationale for the present study. If the manuscript describes a new method, indicate why it is preferable to existing methods.
Note: Tables and Figures: Tables and Figures included in the manuscript that are not part of the Method document itself should be numbered consecutively using the following format: Table 1, Table 2, Figure 1, Figure 2, etc.
Note: Units of Measure: Use the International System of Units (S.I. Units) for reporting measurement results. The Journal prefers the use of the front solidus (/) when expressing units of measure rather than negative index format. For example, use μg/kg not μg kg−1.
Note: Abbreviations, Acronyms, and Initialization: Define non-standard abbreviations (or acronyms, initializations) at first use in parentheses. Care should be taken to limit the use of non-standard abbreviations to improve manuscript readability. Appropriate abbreviations should replace complicated chemical or biological names, but not be used when the reader would substitute the abbreviation for the actual word while reading. Ensure consistency of abbreviations throughout the article. Do not italicize common Latin expressions such as et al., in vitro, via, etc.
A list of standard acronyms and initialization can be found at: http://www.eoma.aoac.org/web%20definition%20of%20terms%20and%20explanatory%20notes.pdf.
Single Laboratory Validation and/or Multi-laboratory (Reproducibility) Study
Subheadings should be written in italic font and may include, but are not limited to, the following:
Study Design
Sample Handling
Test Portion Distribution
Preparation of Test Portions
Test Portion Analysis
Statistical Analysis
Method Document
[start of method document]
AOAC Official Method 20xx.xx (AOAC will fill this in later)
Title of Method
Instrumentation/Technology
First/Final Action 20xx (year approved)
[Applicable to….] Specify utility and limitations on scope of method and other pertinent information
Caution: Statement specifies precautions and possible hazards in carrying out the method, including safety information on applicable equipment, techniques and practices along with safe handling of chemicals, acids, alkalies, microorganisms, and solvents.
Methods are divided into several descriptive sections, including specifications for necessary laboratory apparatus and reagent preparations.
A. Principle (Major headings should be labeled with a capital letter A., B., etc.,)
B. Chemicals and Reagents [This section should include the name of the reagent/chemical, description, manufacturer (city, state, and country) and Model number if available. You may use the registered mark on the first citing after that the copyeditors will delete the registration mark.] Example:
(a) PCR reagent strips.―Two pouches with 12 strips of 8 tubes (2 x 96 tubes) (Manufacturer, city, state, country).
(b) GENE-UP Listeria spp. 2 (LIS 2).—bioMe´rieux, Inc., (Hazelton, MO, USA) Ref 423106 (192 tests) – Store kit at 2–25°C.
Note: Second-level lists are labeled with a bold letter inside parenthesis. You may have a 3rd level if needed. They should be set as follows:
(1)
(2)
(3)
C. Apparatus
This section should include the name of the apparatus, description, manufacturer (city, state, and country) and Model number if available.
Note: You may use the registered mark on the first citing after that the copyeditors will delete the registration mark.
Example:
(a) Ultra-HPLC (UHPLC) system.—Nexera (Shimadzu, Kyoto, Japan) or equivalent LC system consisting of a dual pump system, a sample injector unit, a degasser unit, and a column oven.
C. General Instructions or General Preparation
D. Sample Preparation
E. Determination
F. System Suitability
G. Calculations
Note: Table and Figure numbering: Tables and Figures that appear within the method text should be numbered using the format as follows: 2022.xxA, 2022.xxB, including data supporting the reproducibility of the method (First Action for microbiology, Final Action for chemistry)
[end of method document]
Results and Discussion (or you may separate these)
Results
Level 2 headings should be italic. Examples include:
Calibration Study, Validation Study, Method Developer Studies etc.
Discussion
Conclusion
Acknowledgments
Conflict of Interest
If a conflict of interest does not exist for any of the authors, please include the statement: ‘All authors declare no conflict of interest.’ If any author has a conflict of interest, please state the details of the conflict for each author.
Funding Disclosure (Statement of funding for project, if applicable)
Supplemental Information (if applicable)
Must be uploaded as a separate document clearly labeled.
Supplemental information is available on the J. AOAC Int. website.
References
List references numerically in References section in exact arrangement, punctuation, capitalization, use of ampersand, etc.
Examples:
Journal Article
1. Tuzimski, T., Pieniążek, D., Buszewicz, G., & Teresiński, G. (2019) J. AOAC Int. 102, 23–32. doi: 10.5740/jaoacint.18-0297
Book Chapter
1. Min, D.B., & Ellefson, W.C. (2010) in Food Analysis, 4th Ed. Nielsen, S. (Ed), Springer US, New York, NY, pp 117–132 doi: 10.1007/978-1-4419-1478-1
Book
1. Eitenmiller, R.R., Landen Jr., W.O., & Ye, L. (2007) Vitamin Analysis for the Health and Food Sciences, 2nd Ed. CRC Press, Boca Raton, FL. doi: 10.1201/9781420009750
AOAC Official Method
1. Official Methods of Analysis (2019) 21st Ed., AOAC INTERNATIONAL, Rockville, MD, Method 2016.05. www.eoma.aoac.org [accessed on May 25, 2019]
Standard
1. ISO (2018) Infant formula and adult nutritionals—Determination of vitamin D by liquid chromatography-mass spectrometry (ISO 20636:2018)
Webpage
1. Chromatography Today (2020) Can Chromatography Detect Coffee Adulteration?, https://www.chromatographytoday.com/news/hplc-uhplc/31/breaking-news/can-chromatography-detect-coffee-adulteration/52087 [accessed 19 Nov 2020]
Dataset
1. Authors, Year, Title, Publisher (repository or archive name), Identifier
Figure Captions
Figure captions should be placed after the References.
Ensure that each figure has a caption comprising a brief title and a description.
Tables and Figures
Figures:
Tables:
