October 4, 2023


Determination of Phospholipids in infant formula, adult/pediatric formula and select milk and milk products

AOAC INTERNATIONAL (AOAC) invites method developers to submit methods for consideration and possible evaluation through the AOAC Official MethodsSM program. Prospective methods must be able to measure the amount of Phospholipids found in all forms of infant, adult, and/or pediatric formulas.  Methods with an expanded applicability to select milk and milk products (milk powder, milk protein concentrate, and UHT milk) are highly encouraged for submission.

Quantitative determination of nutritionally relevant total and individual classes of phospholipids (PL), including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), and sphingomyelin (SM), in infant formula and adult nutritionals.

For the purpose of this call for methods, according to the published Standard Method Performance Requirements (SMPR®), AOAC SMPR 2021.017.

Access the SMPR

Method Eligibility

The intended use of the method determined to meet the SMPR and fulfill the ERP recommendations is for Reference Method for Dispute Resolution as designated by Codex as Type II status for phospholipids. Acceptable methods must be reliable, repeatable, and ultimately reproducible when used by trained analysts in accredited laboratories.

Reference the draft SMPR for definitions and the proposed method validation requirements for parameters such as analytical range, limit of quantitation, recovery, repeatability and reproducibility, system suitability and reference materials. Any analytical technique that meets the method performance requirements is acceptable.

Interested method developers must provide their method and data demonstrating that the method can meet the SMPR requirements for determining phospholipids in infant formula and adult/pediatric formula. For those methods with an expanded applicability, sufficient data is required to demonstrate satisfactory method performance for phospholipids in milk powder, milk protein concentrate, and UHT milk.

AOAC Method Submission Requirements and Deadlines

Method authors may submit methods for review to the Official Methods of Analysis (OMA) program.

Official Methods of Analysis (OMA) Program

  • Candidate methods must be submitted in AOAC OMA format, and the data submitted as a manuscript in Journal of AOAC INTERNATIONAL format. No exceptions. Please see the Access to Method Submission Form below as it contains information on requirements for submission, including format information and method safety.
  • Full single laboratory validation required followed by a reproducibility study.
  • Application fees apply for rapid commercial proprietary methods only.
  • Methods and manuscripts must be submitted by specified deadlines.

Submit your method

Submit your method(s) for First Action status consideration. (Email submissions will not be accepted).


For general information or comments on the Official MethodsSM Program, contact Deborah McKenzie.


For general questions on submitting methods for infant formula and adult nutritionals, contact Delia Boyd.

Access SMPR

Click below to view Standard Method Performance Requirements (SMPR):

AOAC SMPR® 2021.017 Standard Method Performance Requirements (SMPRs®) for Determination of Phospholipids in Infant and Adult/Pediatric Nutritional Formula