Matrix studies are the backbone of gluten method validation. AOAC INTERNATIONAL Guidelines for Validation of Qualitative Gluten Methods with Specific Examples – Lateral Flow Devices (SG 2025.001) provides detailed guidance on Probability of Detection (POD) and matrix study design for qualitative methods, ensuring tools perform reliably across real‑world foods and surfaces.
AOAC INTERNATIONAL Guidelines for Validation of Quantitative Gluten Methods with Specific Examples – ELISA Assays (SG 2025.002) builds on this foundation with quantitative requirements, including calibration curve design, residual analysis, LOD/LOQ estimation, recovery expectations, and precision (repeatability and intermediate precision). The guideline also outlines how to rotate gluten sources across matrices and how to prepare spiked and incurred materials.
Together, these guidelines ensure that gluten methods are validated under conditions that reflect actual use — not just ideal laboratory settings.
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