Understanding what a method detects — and what it shouldn’t — is central to gluten testing. AOAC INTERNATIONAL Guidelines for Validation of Qualitative Gluten Methods with Specific Examples – Lateral Flow Devices SG 2025.001 outlines a comprehensive approach to selectivity studies for qualitative methods, ensuring tools detect the correct gluten sources and avoid false positives.
AOAC INTERNATIONAL Guidelines for Validation of Quantitative Gluten Methods with Specific Examples – ELISA Assays SG 2025.002 expands this concept for quantitative methods, detailing structured approaches for evaluating breadth, cross‑reactivity, and interference across diverse matrices. This includes expectations for testing multiple grain sources, identifying interfering ingredients, and reporting cross‑reactive materials to end users.
These harmonized approaches strengthen method reliability and support better decision‑making across the food industry.
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